Supplementary Figure 4: coMESH injection of PSII solution.

(a) PSII dimer solution (non-crystalline): 6.5 mM chlorophyll, in 40% (v/v) glycerol, 100 μm ID × 160 μm OD × 1.5 m long fused silica capillary. The sheath flow capillary was a 30 μm ID × 150 μm OD × 1 m fused silica capillary, primed with 40% (v/v) glycerol, 0.1 M MES pH 6.5, 5 mM CaCl₂. After priming, the atmospheric side of the line was capped off. The outer concentric capillary seen in the figure was a 180 μm ID × 360 μm OD × 5 cm long, tapered fused silica capillary. The sample flow was charged by the needle of the reservoir syringe and was pushed by a syringe pump set to 2 μl/min. The running voltage was 6,000 V and the counter electrode was grounded. (b) A higher resolution image of a PSII solution (non-crystalline) in the protruding coMESH configuration, to emphasize the presence of a wavy irregular appearance at the capillary outer wall, which was possibly a thin coating of liquid from the sheath to aid the meniscus of the sample capillary. (c) A picture of an injector failure in a single capillary (top of the figure behind the scale bar) configuration (large black build-up is frozen PEG-solution at the injector tip and red background is from illumination LED). The solution was a high molecular weight PEG solution. This solution failed in a very similar manner when unassisted by a sister liquor in an extended coMESH configuration.