Supplementary Figure 3: Light path of the inverted light-sheet microscope.
From: Inverted light-sheet microscope for imaging mouse pre-implantation development
Schematic representation of inverted light-sheet microscope light path: lasers (L1, L2), acousto-optical modulator (AOM), clean-up filter (CF), glass plates (GP), kinematic mirrors (KM), dichroic mirrors (DM), optical fiber (F), filter wheel with neutral density filters (FW), polarizing beam splitters (PBS), fixed mirrors (M), beam expander (BE), mechanical shutters (SH), galvanometric scanning mirror (SM), scan lens (SL), tube lenses (TL), excitation light (Ex), illumination objective (IL), imaging objective (IM), motorized filter wheel with emission filters (MFW), emitted light (Em), field stop (FS), relay lenses (RL1, RL2), emission filters (EM1, EM2), sCMOS camera (sCMOS). Position of the EGFP and mCherry channels on the camera chip is indicated in inset.
