Supplementary Figure 3: Modulation of cytokine and chemokine gene expression in COPD small airway chips using a BRD4 inhibitor.
From: Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro
Quantitative real-time PCR analysis of the effects of budesonide and BRD4 inhibitor (applied as described in Fig. 5) on expression of IL-8, MCP-1, IL-6 and GRO-α genes in lung blood microvascular endothelial cells lysed in situ within the microvascular channel of the chips (unpaired Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001; data represent mean ± s.e.m (compared to untreated) from two of the donors studied in Fig. 5, with 1–2 biological replicates per condition; n = 3).
