Supplementary Figure 4: Distribution of predicted expression levels in the constrained optilib. | Nature Methods

Supplementary Figure 4: Distribution of predicted expression levels in the constrained optilib.

From: Predictable tuning of protein expression in bacteria

Supplementary Figure 4

The 40,526 oligos designed to change expression of all E. coli genes. The oligos were designed with the constraint to not modify the coding sequence of overlapping genes. Even with the constraints, it was possible to design sequences with predicted expression close to the intended target value for most sequences.

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