Supplementary Figure 6: Analysis of the endogenous human GAPDH complex, fractionated from HeLa S3 cells.
From: An informatic framework for decoding protein complexes by top-down mass spectrometry
The 3-tier tandem mass spectrometric data for the detected complex is illustrated with the (a) MS1, (b) MS2 (multiple forms shown in inset) and (c) graphical fragment ion map of the ejected monomer that corresponds to P04406-1, the canonical GAPDH isoform (E-value of 1 × 10−23, C-score of 3). The modification to Cys152 was localized as discussed in the supplementary discussion and is highlighted in orange. (d) The homotetrameric MPC identified from a database search combining MS1, the singly-modified MS2, and MS3 information that was assigned a very high MPC-score of 2,924.
