Supplementary Figure 6: Phototoxicity of TAPs on FAP-expressing HEK cells.
From: A genetically targetable near-infrared photosensitizer
(a) Dose dependent cytotoxicity by MG-2I on HEK cells expressing cell surface dL5** with various illumination intensity (1 minute illumination time with 25%, 40%, 50%, 60%, 70%, 80%, 90%, 100% laser power) or illumination duration (50% laser power with 1, 2, 3, 4, 5 minute illumination time) using confocal microscopy (40× objective, 640 nm). (n = 3, mean and S.E.M. plotted) (b) Phototoxicity was suppressed only by sodium azide, but not catalase or SOD, indicating the cytotoxicity was singlet oxygen mediated. ROS quenchers were added to cells 5 minutes prior to illumination and allowed 5 minutes rescue after illumination before Live/Dead staining as above. (n = 4, mean and S.E.M. plotted)
