Supplementary Figure 8: Detection of superoxide species from FAP-TAPs ROS-generating cascade by DHE.
From: A genetically targetable near-infrared photosensitizer
(a) Wild type HEK cells or cells expressing FAP with a nuclear localization signal (NLS-dL5** HEK) were first labeled with 400 nM of dye, and then 2 μM of DHE was added to cells 10 minutes prior to 2 minutes of illumination (60× objective, 640 nm, 2.43 W cm-2). Imaging was carried out immediately after photosensitization. (b) Mean fluorescence intensity change after illumination of each group. (n = 6, mean and S.E.M plotted. Scale bar = 10 μm and applied to all images, 488 channel: 488 nm excitation and 535/25 nm emission filter).
