Supplementary Figure 10: In vivo assessment of collateral damage using larvae transgenic zebrafish; apoptosis was restricted to FAP-TAPs−targeted cells.

Fish were fixed 24 hpi and stained using a whole mount TUNEL assay and immunolabeling of the mCer3 with anti-GFP antibodies and a secondary alexa555 conjugated donkey anti-rabbit antibody. (a) Representative imaging of orthgonal view and sphere reconstruction of TUNEL/mCer3 fluorescence. (b) mCer3⁺ cell count, fewer mCer3⁺ cells are seen in MG-2I 12 min group (*). (c) TUNEL positive cell count, green represents TUNEL signals that colocalized with mCer3 signals; black represents TUNEL signals that were not associated with mCer3⁺ cells. (NS for all groups) (d) TUNEL/mCer3⁺ ratio, MG-2I with 12 min illumination induced 31.5% (**) cell death of mCer3⁺ cells compared to MG-ester with 12 min group. A reduced fraction of mCer3⁺ cells are killed (24.8%) when 4 min illumination is applied (*). Scale Bar = 10 µm and applied to all images, n = 8, mean and S.E.M plotted. One-way ANOVA, Tukey post hoc tests were performed with multiple comparisons of mean for each group. Paired t test was used for comparisons between specific death and non-specific death. *P < 0.05, **P < 0.01.

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