Supplementary Figure 1: Characterization of FAP-TAPs binding and spectroscopic properties.
From: A genetically targetable near-infrared photosensitizer
(a) Dissociation equilibrium constant (Kd) of MG-ester–dL5** and MG-2I–dL5**, where 5 nM of protein was complexed with various concentrations of dyes. Fluorescent intensity was measured, normalized and analyzed by a one-site binding ligand depletion model. (n = 4, mean and S.E.M. plotted) (b) Fluorescent quantum yield measurement of MG-2I–dL5** using Cy5 as standard (emission spectra were integrated, normalized to the maximum value and plotted against absorbance at 600 nm).
