Supplementary Figure 5: Light box (built by M.P.B.).
From: A genetically targetable near-infrared photosensitizer
The box consists of a heatsink cooled 100 W deep-red LED array (LED World, 660 nm, 6000 lm specified) powered by a 24 V power supply, controlled with a CraLab enlarger timer. A cooling fan blows air through the chamber to prevent heating of the specimens during prolonged exposures. This device can also be inverted for illumination of fish, and has relatively flat-field illumination (< 5% variation) across a 96-well plate area with 0.089 W cm-2 power density (24 nm Full Width at Half Maximum). A thermometer can monitor the temperature inside the chamber to ensure samples are kept at or below physiological temperatures.
