Supplementary Figure 1: k-mer coverage histograms.
From: TruSPAdes: barcode assembly of TruSeq synthetic long reads
Histograms of k-mer coverage (k = 55) for the E. coli standard isolate dataset from Bankevich et al.16 (a), the E. Coli MDA-amplified single cell dataset from Bankevich et al.16 (b), one of the barcodes of TSLR data (c) and a single 10 Kb long fragment of a barcode (d). Conventional assemblers select a coverage threshold to separate correct from erroneous k-mers. The histogram for data from the standard isolate features a smaller peak on the left (formed by largely erroneous k-mers with low coverage) and a larger peak on the right (formed by largely correct k-mers with high coverage). Thus, one can choose a proper threshold that separates correct from false k-mers38. However, for both MDA and TSLR, there is no threshold separating correct and false k-mers.
