Supplementary Figure 6: Single staining of U2OS cells with MitoTracker Orange.
From: Multi-target spectrally resolved fluorescence lifetime imaging microscopy
(a) Overall fluorescence intensity image. Using sFLIM three slightly different reference patterns (f,g) can be isolated, and three different cellular structures visualized: (c) mitochondria (magenta), (d) endosomes (green), and (e) unspecific staining, accumulated inside the nucleoli (yellow). (b) sFLIM composite image. The brightest pixels belong to 3,718 (a) / 3,275 / 2,496 / 1,223 (c–e) photons. Scale bars, 10 µm.
