Supplementary Figure 7: Testing the accuracy of the pattern-matching algorithm. | Nature Methods

Supplementary Figure 7: Testing the accuracy of the pattern-matching algorithm.

From: Multi-target spectrally resolved fluorescence lifetime imaging microscopy

Supplementary Figure 7

Fluorescence decay patterns of three different secondary antibodies labeled with Cy3, TRITC, and TexasRed, respectively. The decays are not mono-exponential and show average lifetimes of 1.12 ns, 2.30 ns, and 3.36 ns. These decay patterns (without spectral information) were used to simulate signals of varying contributions of the patterns.

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