Supplementary Figure 10: Appropriate average patterns.
From: Multi-target spectrally resolved fluorescence lifetime imaging microscopy
We created different reference patterns from singly- and multi-stained samples to analyze Golgi, nascent DNA and F-actin, all labeled with ATTO 488 (Fig. 4). We then calculated average patterns and standard deviations of all patterns that we classified to be appropriate, i.e. that comply with the requirements described in the Online Methods. Black lines represent average patterns for giantin/Golgi (a), phalloidin/F-actin (b), and EdU/DNA (c). Grey areas illustrate the average patterns plus and minus their standard deviation. 4/6/3 patterns have been used (a–c). Colored lines show examples of inappropriate patterns.
