Supplementary Figure 12: Comparison of results when two independently acquired sets of reference patterns are used.
From: Multi-target spectrally resolved fluorescence lifetime imaging microscopy
(a,b) Reference patterns, in magenta / green / blue as used in Fig. 4 (3x ATTO 488), and in brown / dark green / dark blue a second set of patterns, generated from one singly-stained sample (giantin/Golgi) and from singly-stained parts of two multi-stained images (phalloidin/F-actin and EdU/DNA). (c–f) Results obtained for the second set of pattern (Golgi, F-actin, DNA, Composite), and (g–i) pixel intensity differences in comparison to Fig. 4 (Golgi, F-actin, DNA). Here green indicates an increase and magenta a decrease in signal intensity. Numbers in brackets represent the photon counts of the brightest pixels. The mean of the absolute value of the difference is 15.0, 26.7, and 15.8 photons per pixel (g–i). (j) Mere intensity image. Scale bars, 10 µm.
