Supplementary Figure 3: Using less information for sFLIM analysis.
From: Multi-target spectrally resolved fluorescence lifetime imaging microscopy
U2OS cell labeled with five fluorophores excitable at 640 nm (Supplementary Fig. 2). (a) sFLIM image using the total information available (spectral and lifetime information). (b) Fluorescence induced by 485 nm and 532 nm lasers was ignored in data analysis and only the fluorescence from the most appropriate laser at 640 nm was used. (c) Only the spectral information (i.e. spectral un-mixing), and (d) only the lifetime information upon irradiation at 640 nm was used (i.e. FLIM). Scale bars, 10 µm.
