Supplementary Figure 1: Validation of different PLAYR insert systems used for multiplexed detection of transcripts.
From: Highly multiplexed simultaneous detection of RNAs and proteins in single cells
The three transcripts ACTB, GAPDH, and PPIA were detected using 2 probe pairs per gene on 7 different insert systems used for multiplexed detection of transcripts. Rolling Circle Amplification was carried out for 30min, 1h, 2h, 4h or over night (16h) and samples were analyzed by mass cytometry.
