Supplementary Figure 4: Detection of specific transcripts in single cells by flow cytometry using multiple probe pairs.
From: Highly multiplexed simultaneous detection of RNAs and proteins in single cells
a) Detection of CD10 and CD3E by PLAYR in three replicates. Jurkat and NALM-6 cells were incubated with the indicated number of probe pairs and analyzed by flow cytometry. The expression of CD3E and CD10 is specific to Jurkat and NALM-6 cells respectively. b) The intensity of PLAYR signals depends on the distance between PLAYR probe binding sites on a target transcript. Multiple adjacent probe pairs spanning a transcript were designed and tested in all possible pairwise combinations. The x-axis represents the distance between each pair of probes, and the y-axis represents the ratio between the signal obtained with a given combination and the signal obtained with the corresponding adjacent cognate probe (i.e., the one that was originally designed to be used in the pair).
