Supplementary Figure 6: Average coverage and methylation rate of the Arabidopsis thaliana genome as measured with PstI-epiGBS data.
From: epiGBS: reference-free reduced representation bisulfite sequencing
Per cytosine, average coverage and methylation rate were calculated per 1MB windows for all four Arabidopsis lines sequenced in PstI epiGBS for the Arabidopsis reference genome TAIR10 on chromosome 1-5. Only positions covered by at least 10 reads were taken into account. Average methylation is higher in centromeric regions but per cytosine coverage is lower in these regions, reflecting sensitivity of PstI to 5mC methylation.
