Supplementary Figure 2: General features of DNase I sequence preference.

(a) Relative cleavage preference of all 4,096 hexamers with respect to the median hexamer as determined by deep sequencing (~100 million tags) of a DNase I digestion of deproteinized DNA from human IMR90 cells (data from ref.). (b) Biased hexamers contribute disproportionately to total DNase I cleavages for both naked DNA and chromatin (regulatory T cells cleavages mapping within DHS) when compared to the 36 bp mappable genome. Shown is the cumulative fraction all mappable positions or sequencing tags within respect to their hexamer context. Hexamers are ranked by decreasing cleavage preference as in a.