Supplementary Figure 3: Influence of cell-cycle correction on data clustering and GO enrichment
From: Diffusion pseudotime robustly reconstructs lineage branching
a,b) The total count of transcripts from 2047 heterogeneous genes per day. a) log-normalized counts before cell-cycle correction. b) log-normalized counts after cell-cycle correction. c) Fit the CV2-mean relation according to Brennecke et al [11] to a pure RNA control and d) superimpose these technical genes with endogenous genes. e) Variance decomposition according to the identified latent variables. f) Detailed variance decomposition sorted by technical noise contribution.
