Supplementary Figure 12: IL-2 and perforin protein levels were unremarkable in muscles injected with AAV9-Cas9-VPR-gRNAs.
From: A multifunctional AAV–CRISPR–Cas9 and its host response
Mice were targeted with gRNAs set 1 (7 gRNAs against Mstn, Fst, Pd-l1 and Cd47) or with gRNAs set 2 (3 gRNAs against Mstn and Fst), all at 4E12 vg total of 1:1 AAV9-Cas9N-gRNAs:AAV9-Cas9C-VPR (n = 3 mice per condition, including data presented in Figure 3c). All injections include 1E11 vg of AAV9-turboRFP to demarcate transduction. Scale bar, 500 μm.
