Supplementary Figure 5: Whole-mount epifluorescence images from neonatal mice injected intraperitoneally with AAV9-Cas9-gRNAs (5E11 vg) targeting the 3×Stop cassette and controls.
From: A multifunctional AAV–CRISPR–Cas9 and its host response
Numerous tdTomato+ cells were observed in mice injected with AAV9-Cas9-gRNAs targeting the genomic 3×Stop cassette (Td5+Td3 and TdL+TdR), but not in negative control vehicle-injected mice, indicating that fluorescence activation resulted from 3×Stop excision. TdTomato+ cells were also observed, at low frequencies, in mice injected with AAV9s encoding two gRNAs both targeting one side of the 3×Stop cassette (AAV9-Cas9-gRNAsTd5+TdL or AAV9-Cas9-gRNAsTd3+TdR), suggesting the rare introduction of large deletions that removed the 3×Stop terminators. All injected mice are shown. Gray, tdTomato; scale bar, 5 mm.
