Figure 4: Structural insight into a predicted ENTH–Ub interface.
From: A bacterial genetic selection system for ubiquitylation cascade discovery
(a,b) Coexpression of ENTH domains from Danio rerio Epn1 (zebrafish, a) or Saccharomyces cerevisiae Ent1 (yeast, b) with the ubiquitylation apparatus in the bacterial selection system. Bacterial spots were seeded and visualized as in Figure 1. Complete, coexpression of pND–Ub, including E1 and yeast Ubc4 and pCD–ENTH domain. (c,d) Structures of the zebrafish and yeast epsin1 ENTH domains. The 2mFo-DFc sigma-A maps of the fish and the yeast proteins at 2.2 and 1.8 σ, respectively, of the refined models. (e) Superimposition of the fish and the yeast ENTH structures on top of the STAM1-VHS–Ub complex (pdb code 3LDZ). Average Cα r.m.s. deviations are 1.5 Å and 1.7 Å, respectively. (f) Zoomed-in view into crystal structure of the VHS–Ub interface (3LDZ). (g–h) Refined models of the ENTH–Ub complexes. (i,j) Zoomed-in view into the ENTH–Ub interfaces of the models.
