Supplementary Figure 2: Architecture and sequence of the linkers connecting the DHFR fragments to ubiquitin and to ubiquitylation target
From: A bacterial genetic selection system for ubiquitylation cascade discovery
Schematic illustration of the constructs used in the selection system. pND-Ub vectors contain a N-Terminal DHFR fragment fused through the flexible linker (linker 1) to Ub. In the pCD-Sub the C-Terminal fragment of the DHFR was fused through the flexible linker (linker 2) to the His6-MBP-substrate or directly to the substrate.
