Supplementary Figure 6: Systematic imaging processing of ATAC-see
From: ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing
a, Schematic of image processing workflow of making mitochondria masks from ATAC-see and define the bright area of ATAC-see signal in the nucleus. b, Analysis of two different ATAC-see patterns in CD4+ T cells. The two plots in the upper panel represent the radial distribution of ATAC-see signal intensity and DAPI signal intensity in two different groups of CD4+ T cells. The blue line shows the ATAC-see signal intensity is low at the nucleus periphery, the green line illustrates the ATAC-see signal form a rim structure at the nucleus periphery (“Cap pattern”) and the red line indicates the means of all signal intensity. The line plot in the middle panel shows the ratio of ATAC-see signal intensity and DAPI signal intensity. The scatter plot in the lower panel represents the correlation of ATAC-see signal intensity and DAPI signal intensity among the population cells. The red lines in the plot represent the average values of single intensity from all groups.
