Supplementary Figure 9: Immunostaining in human neutrophils and NETosis
From: ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing
a, Immunostaining of epigenetic markers in human neutrophil. RNAPII Ser-5 P= RNA polymerase II phosphorylation ser-5. Multi images (n>5) were taken with independent replicates in each staining. b, The representative images show DAPI staining of control, PMA and PAD4 inhibitor (PAD4i) treated human neutrophils. Scale bar=2 μm. c, The bar graph presents the quantification of NETosis in PMA and PAD4 inhibitor treated human neutrophils based on DAPI staining, Error bar= standard deviation; n=60x2 for each condition. d, Representative whole frame images of H3 citrullination staining in control, 5 h PMA treated and 5 h PAD4 inhibitor (PAD4i) treated human neutrophils. Multi images (n=5x2) were taken in each condition. Scale bar=2 μm.
