Supplementary Figure 8: Mutagenesis of protein coding and promoter regions with AID*Δ.

a) Gene diagrams for each locus n indicate the position of the respective sgRNAs. Each sgRNA was infected into cells expressing dCas9 and MS2-AID*Δ in duplicate. Shown are graphs of the enrichment of mutations at positions relative to the PAM at each of the loci. b) sgRNAs targeting either GFP or endogenous loci were integrated via two independent infections (n=2 for each sgRNA) into cells expressing dCas9, MS2-AID*Δ, GFP, and mCherry. Graphs showing the frequency of alternative alleles at each base position relative to the PAM of the sgRNA are shown. c) Box plot indicating the range of frequency of mutated reads over the 100bp region for 30 sgRNAs is shown (n=60). The lines represent 1.5 times the interquartile range. Median value is indicated above graph and represented by the center line. d) Graph of the percentage of all possible single base changes observed for AID*Δ targeted with sgRNAs (described in Fig. 4a,c) in a 21bp sliding window. Single base changes with a frequency above the estimated noise were counted over a 21bp window beginning at the indicated position relative to the PAM, and the measured fraction of all possible changes is reported for each window. Box plots at each position are shown summarizing the distribution observed over all sgRNAs samples (n=60). The center line represents the median. The whiskers represent 1.5X the interquartile range, and outliers are indicated as dots.