Supplementary Figure 9: Simultaneous mutation of multiple sites.
From: Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
sgGFP.10 and sgmCherry.1 were integrated (n=3 for each pair of sgRNAs) separately or in combination into cells expressing dCas9, MS2-AID*Δ, GFP, and mCherry. The GFP and mCherry fluorescence of the cells were measured. The scatter plots of the flow cytometry for each of the samples are shown (left). A graph summarizing the percentage of GFP negative or mCherry negative cells is shown (top left). In the bottom left panel, a graph displaying the percentage of cells that have neither GFP nor mCherry is shown. Error bars indicate standard error.
