Supplementary Figure 2: On-target mutagenesis using CRISPR-X with limited off-target effect.
From: Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
a) Cells were infected as independent replicates (n=3) with indicated combinations of MS2-AIDΔ or MS2-AIDΔDead and sgGFP.1 or sgNegCtrl and the GFP and mCherry fluorescence of the cells was measured by flow cytometry as a proxy for mutation rate. Shown are the scatter plots from flow cytometry and graphs summarizing the non-fluorescent populations. Error bars represent standard error. b) GFP and mCherry loci of the infected cells were sequenced and enrichment of mutation was calculated at each base position for three replicate experiments.
