Supplementary Figure 3: CRISPR-X tiling of GFP locus.
From: Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
a) Map of sgRNAs tiling the GFP locus. b) sgRNAs targeting GFP were integrated via independent infection (n=3) into cells expressing dCas9, MS2-AIDΔ, GFP, and mCherry. Enrichment of mutation relative to the position of the PAM of the sgRNAs is shown. The direction of transcription was defined as the positive direction as indicated by the arrow. c) Enrichment of mutations at each base position is shown for three replicates of each sgRNAsgGFP.2-12. The relative position of the sgRNA is indicated above each graph. d) A box plot indicating the frequency of mutated reads observed in the respective hotspot of each sgRNA is shown. The median value for the conditions is listed above each sample and indicated by the center line. The box plot lines represent the 1.5 of the interquartile range.
