Supplementary Figure 5: Identifying bortezomib resistant mutations in PSMB5.
From: Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
a) Enrichment of mutations at each base position for the PSMB5 and Safe-targeted libraries (corresponding to Fig. 3b) is shown. b) A replicate experiment was performed for directed evolution of bortezomib-resistant PSMB5 mutations (see Fig. 3). Enrichment of mutations at each base position after selection with bortezomib for both the PSMB5 and Safe-targeted libraries is shown. c) Graphs of mutation enrichment are shown for individual exonic loci of PSMB5 for the second replicate. Mutations that were enriched beyond the 20-fold cutoff (dashed black line) are observed in Exons 1, 2, and 4.
