Supplementary Figure 8: Relaxation analysis of DMPC/DMPG (3:1) phospholipids in cNW and ΔH5 nanodiscs.
From: Covalently circularized nanodiscs for studying membrane proteins and viral entry
(a) 1H-NMR T1 relaxation times of DMPC/DMPG lipid signals in empty ΔH5 (blue) and cNW9 (red) nanodiscs. (b) 1H-NMR T2 relaxation times of DMPC/DMPG lipids signals in empty ΔH5 (blue) and cNW9 (red) nanodiscs (c) Chemical structure of DMPC and DMPG.
Except the DMPC γ methyl group located outside the bilayer region, the lipids in cNW9 show shorter T1 and T2 times consistent with smaller nanodisc size and dynamics indicative of the more restrictive circularized cNW9 belt. Measurements were acquired at 45ºC on a Bruker 500-MHz spectrometer using inversion recovery for T1 and CPMG refocusing train for T2.
