Supplementary Figure 11: Single-particle EM analysis of negatively stained VDAC-1 in cNW9 nanodiscs. | Nature Methods

Supplementary Figure 11: Single-particle EM analysis of negatively stained VDAC-1 in cNW9 nanodiscs.

From: Covalently circularized nanodiscs for studying membrane proteins and viral entry

Supplementary Figure 11

(a) Representative EM image of VDAC-1 in cNW9. Right. Enlarged image of the rectangular area in panel a, showing one VDAC-1 channel per nanodisc. Images were collected on a Tecnai T12 electron microscope (FEI) operated at 120 kV. (b) Representative two-dimensional (2D) class averages of VDAC-1 in cNW9 nanodisc, showing distinct views (top, tilted and side views). (c) Cartoon representation of VDAC-1 in a cNW9 nanodisc. Scale bars, 100 nm (a) and 20 nm (b)

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