Supplementary Figure 12: Comparison of the quality of NMR spectra of VDAC-1 in ΔH5 and cNW9 nanodiscs. | Nature Methods

Supplementary Figure 12: Comparison of the quality of NMR spectra of VDAC-1 in ΔH5 and cNW9 nanodiscs.

From: Covalently circularized nanodiscs for studying membrane proteins and viral entry

Supplementary Figure 12

(a) 1H-15N TROSY HSQC recorded at 45°C of 100 uM 15N-2D-labeled VDAC-1 in ΔH5 3:1 DMPC:DMPG nanodiscs acquired overnight on a 600-MHz spectrometer. (b) 1H-15N TROSY HSQC recorded at 45°C of 100 μM 15N-2D-labeled VDAC1 in cNW9 3:1 DMPC:DMPG nanodiscs acquired overnight on a 800-MHz spectrometer. The spectral quality and sample stability are greatly improved by using cNW9 as compared to ΔH5 nanodiscs.

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