Supplementary Figure 13: Cryo-EM images of poliovirus plus CD155-decorated 50-nm nanodiscs.
From: Covalently circularized nanodiscs for studying membrane proteins and viral entry
(a) Representative cryo-EM images of poliovirus plus CD155-decorated 50-nm nanodiscs in vitreous ice. Scale bar represents 100 nm. (b) Representative cryo-EM images of poliovirus-nanodisc complexes showing different intermediate states. Scale bars, 100 nm (a) and 50 nm (b).
We find it unlikely that the putative pore in the nanodisc is a hexametric arrangement of CD155 since we used only the ectodomains of the receptor linked to lipid molecules via His tags. Scale bars, 100 nm (a) and 50 nm (b)
