Supplementary Figure 3: Characterization of cNW50 by MS/MS confirms the ligation of the C terminus to the N terminus.
From: Covalently circularized nanodiscs for studying membrane proteins and viral entry
Spectrum of a tryptic fragment of cNW50 showing the ligation of the C-terminal motif (LNTQLPGTG-His6) to the N-terminal residues (GSTFSK). Expected masses for b and y ions along with the peptide sequence are listed in the table. The b and y ions that were positively identified in the MS/MS spectrum are highlighted in blue and red. The amino acid sequence of linear NW50 (after TEV cleavage) is shown at the top.
