Supplementary Figure 6: Effects of multiplicity of infection (MOI) in the cell library on the AsCpf1-induced indel frequencies.
From: In vivo high-throughput profiling of CRISPR–Cpf1 activity
Three different cell libraries with different MOIs (0.4, 2, and 10) were generated by modifying the transducing amount of guide RNA-expressing lentivirus and AsCpf1 was lentivirally delivered into these cell libraries. Indel frequencies were measured 5 days after AsCpf1 delivery. Number of analyzed pairs (n) = 273 per group. (a) AsCpf1-induced indel frequencies in the cell libraries with different MOIs. Error bars represent s.e.m. **P < 0.01, ***P < 0.001, ANOVA followed by Tukey’s post hoc test. (b-d) Correlation between indel frequencies in cell libraries with different MOIs.
