Supplementary Figure 8: Epitope mapping of anti-LexA-H3-Gcn5wt-HA intrabodies.

(a) Liquid beta-galactosidase assay shows how ScFv-58F is the only intrabody that is able to recognize the screening bait exclusively. The right panel gives an idea of the interaction strength, which is proportional to color intensity. Values are normalized by subtraction of the negative control (ctrl- in the left figure) and reported as bar chart on the right. Y1 is an anti-LexA intrabody, used as the positive control (ctrl+). (b) Beta-galactosidase filter assay demonstrates the same interaction pattern observed with the liquid assay. Background levels of LacZ due to auto-activation are more evident in the filter assay than in the liquid assay (see the negative control of the H3-Gcn5wt panel).