Supplementary Figure 13: Full-length western blot membranes. | Nature Methods

Supplementary Figure 13: Full-length western blot membranes.

From: Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies

Supplementary Figure 13

(a, b) Membranes from which right panels of Supplementary Figure 9a are derived, showing inputs of in vitro acetylated/non-acetylated recombinant H3 Histone. In (b), anti-His PENTA by Qiagen cross-reacts with BioRad Dual Color marker (cross-reaction seen also in other blots at the dilution of 1:1000 – 1:2000 and for longer exposures). (c) Blot membrane from which Figure 2c is derived. First lane= BioRad Dual Color. From the fifth lane, samples are not relevant to the experiment, and were used as internal controls. (d) Membrane from which Figure 2c is derived. Last lane is another yeast extract used as internal control. (e, f) Western blot membranes of experiments shown in Supplementary Figure 9b. Lanes 5-7 are non-relevant for the experiment. For all gels, BioRad Dual Color marker was used.

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