Supplementary Figure 10: Assessment of peptide purity and side product profile
From: Building ProteomeTools based on a complete synthetic human proteome
(a) Using an unspecific MaxQuant search with the “depended peptides” option enabled, a synthesis tree view of the peptide ESQLKDLEAENRR was constructed that displays the estimated relative yield of the desired full length peptide product (85%) as well as other side products in the synthesis. (b) Same as panel (a) but for the peptide LVFVDAVAFLTGK what displays an estimated relative yield of the desired full length peptide product of 52%. The tree lists all identified truncation and by-products and their relative contribution to the entire signal intensity attributable to these molecular species. By-products with less than 1% estimated yield are omitted from the visualization fro clarity. Annotation from bottom to top: Peptide sequence identified, potential modification, mass error to annotated modification in ppm, delta mass compared to the full length peptide (e. g. mass of missing amino acid or additional protection group) and percentage of the total intensity of the identified synthesis products. The correct full length product is marked in green.
