Supplementary Figure 6: HCD fragmentation spectra of YYLIQLLEDDAQR with Orbitrap readout at different collision energies. | Nature Methods

Supplementary Figure 6: HCD fragmentation spectra of YYLIQLLEDDAQR with Orbitrap readout at different collision energies.

From: Building ProteomeTools based on a complete synthetic human proteome

Supplementary Figure 6

Fragmentation spectra of the peptide YYLIQLLEDDAQR for all six HCD methods used in this study (normalized collision energies of NCE 20, 23, 25, 28, 30, 35 respectively). All annotated spectra are the best identification from Andromeda (i.e. highest score) for the respective fragmentation mode.

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