Supplementary Figure 9: Validation of the FLIP cassette by insertion in the endogenous TP53 gene of hiPSC cells.
From: One-step generation of conditional and reversible gene knockouts
Detection of correctly targeted human TP53 (hTP53) in human induced pluripotent stem cell (hiPSC) clones (a-c). The FLIP cassette containing a resistance gene was inserted into the exon 4 of hTP53 (a). Detection of correctly integrated 5’ and 3’arms by PCR in ESC clones targeted with the FLIP cassette (b). The clones H4, C4, F4 are correctly targeted. Sequencing results of the second allele of the hTP53 gene allow identification of insertion and deletion. Clone C4 has an 11bp deletion and clone F4 has a 13bp insertion (c).
