Supplementary Figure 10: Representative transfection efficiency analysed by flow cytometry 48h post transfection in mESCs.
From: One-step generation of conditional and reversible gene knockouts
(a) Mouse embryonic stem cells (mESCs) were transfected with an eGFP expressing plasmid using Lipofectamine-2000 and analysed 48h post transfection. Left panel shows the untransfected control and the right panel the transfected cells. The middle panel contains a histogram displaying the percentage of eGFP positive cells of the transfected population (blue line) compared to untransfected population (red line).
(b) Representative images of eGFP transfected mESCs 48h post transfection. Left panel brightfield image of transfected cells, middle panel GFP expression of transfected cells, and right panel as an overlaid image. Scale bar 1000 μm.
