Supplementary Figure 12: Insertion of the FLIP-FlpE cassette into exon 16 of Apc in mouse small intestinal organoids. | Nature Methods

Supplementary Figure 12: Insertion of the FLIP-FlpE cassette into exon 16 of Apc in mouse small intestinal organoids.

From: One-step generation of conditional and reversible gene knockouts

Supplementary Figure 12

(a) Schematic image showing the insertion of the FLIP-FlpE cassette into the exon 16 of Apc.

(b, c) PCR detection of FLIP-FlpE cassette insertion in the Apc locus in mouse intestinal organoids. The correctly targeted clone (A8) is positive for 5’ and 3’arm genotyping PCR reactions (b). Exon PCR and sequencing confirmed that the remaining allele has 1 bp deletion (c).

(d) Representative images of control, and ApcFLIP-FlpE/- mouse intestinal organoids with and without 4-hydroxy tamoxifen (4-OHT). Top row in 4x, Scale bar 500 μm and bottom row 10x. Scale bar 200 μm.

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