Supplementary Figure 10: Rfree – Rwork as a function of refinement cycle.
From: Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED
The R-factor gap is always less than 6% and the variation is generally within ±1% after the first few cycles. The variation is greater for TGF-βm–TβRII, thaumatin, and thermolysin, which are the lowest-resolution structures in this study. (Left) tau peptide (dashed black curve), lysozyme (solid orange curve), TGF-βm–TβRII (solid blue curve), and xylanase (dashed green curve). (Right) thaumatin (solid black curve), trypsin (dashed orange curve), proteinase K (solid blue curve), and thermolysin (dashed green curve). All refinements were performed using phenix.refine [Afonine et al. (2012) Acta Crystallogr D Biol Crystallogr 68, 352–367].
