Supplementary Figure 8: Transcript Cutoff For Species-Mixing Validation
From: Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
We sequenced two arrays (a & b) to confirm single-cell resolution and minimal cross-contamination between mouse and human cells. We called cells by plotting the cumulative distribution of transcripts and making a cutoff at the elbow in the curve. In the first experiment (a), which was used to validate our single-cell resolution, we shallowly sequenced the array and made the cutoff at 2,000 transcripts. In the second experiment (b), where we sequenced the array deeply to allow a competitive comparison to Drop-Seq, we made our cutoff at 10,000 transcripts.
