Supplementary Figure 10: Mapping Lineage Defining Transcripts To PBMC Clusters
From: Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
(a) Clusters identified through graph-based clustering (Methods) correspond to major immune cell populations. (b,e) CD4 T cells are characterized by expression of CD3D and T-cell receptor expression without pronounced expression of cytoxic genes NKG7 and PRF1. (c,f) CD8 T cells are defined by expression of NKG7 and PRF1. (d,g) Monocytes are defined by expression of cathepsin B (CTSB) and SOD2. (e) Natural killer cells are characterized by expression of cytotoxic genes in the absence of T cell receptor expression. (h) B cells are marked by elevated expression of MS4A1 (CD20) transcripts. (i) Dendritic cells are enriched for expression of BIRC3.
