Supplementary Figure 11: Heatmap Of PBMCs
From: Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
(a) Genes enriched in each cluster were identified using an “ROC” test in Seurat, comparing cells assigned to each cluster to all other cells. A heatmap was constructed using enriched genes found to define each cluster. One cluster of 602 cells that demonstrated exclusive enrichment of mitochondrial genes was removed as these likely represent low-quality or dying cells. (b) We generated a t-SNE projection of 4,296 cells with greater than 10,000 reads, 1,000 transcripts, 500 genes, and 65% transcript mapping. We removed a total of 602 cells from the final analysis found to be strongly enriched for expression of mitochondrial genes. The remaining 3,694 cells form distinct clusters enriched for lineage-defining that distinguish cells types from one another.
