Supplementary Figure 13: Comparison Of Human PBMC Gene And Transcript Capture With Other Massively-Parallel scRNA-Seq Methods
From: Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
(a) Comparison of transcript capture (top) and gene detection (bottom) between Seq-Well and 10X Genomics within PBMC cell types prior to downsampling (colored as in Figure 2; Center-line: Median; Limits: 1st and 3rd Quartile; Whiskers: +/- 1.5 IQR; Points: Values > 1.5 IQR). Among B cells (orange), an average of 1,315 genes and 3,632 transcripts were detected using Seq-Well and an average of 710 genes and 1,910 transcripts were detected in 10X Genomics data. Among CD4 T cells (blue), an average of 861 genes and 2,444 transcripts were detected using Seq-Well and an average of 815 genes and 2,370 transcripts were detected in 10X Genomics data. Among CD8 T cells (yellow), an average of 885 genes and 2,574 transcripts were detected using Seq-Well and an average of 809 genes and 2,029 transcripts were detected in 10X Genomics data. Among Monocytes (green), an average of 1,288 genes and 3,568 transcripts were detected using Seq-Well and an average of 974 genes and 2,835 transcripts were detected in 10X Genomics data. Among NK cells (red), an average of 902 genes and 2,338 transcripts were detected using Seq-Well and an average of 907 genes and 1,943 transcripts were detected in 10X Genomics data. (b) Transcript capture (top) and gene detection (bottom) upon downsampling of Seq-Well data to an average read depth 69,000 reads per cell (Center-line: Median; Limits: 1st and 3rd Quartile; Whiskers: +/- 1.5 IQR; Points: Values > 1.5 IQR). Upon downsampling, in Seq-Well, an average of 1,048 genes and 3,103 transcripts were detected among B cells, 735 genes and 2,221 transcripts among CD4 T cells, 763 genes and 2,353 transcripts among CD8 T cells, 1,052 genes and 3,105 transcripts among monocytes, and 789 genes and 2,041 transcripts among NK cells.
